Adeno-associated Virus (AAV) Service
AAV is a member of the Parvoviridae family. It is a non-enveloped DNA virus that plays an important role in mediating infection. Recent studies have also shown that recombinant AAV has a higher transduction frequency and can cause minimal immune responses in various types of cells. In particular, the transferred gene can maintain stable expression in human cells. The application of AAV in gene therapy has created a demand for high-titer and purified AAV reserves. Many advanced technologies have been developed to improve the purification of AAV. As mentioned above, a packaging plasmid containing all the requirements for AAV amplification and packaging has been generated. The data shows that the plasmid can effectively produce high-titer adenovirus-free AAV.
Overall Solutions
CD BioSciences, with the assistance of our professional scientific team, CD BioSciences provides a series of AAV services, including but not limited to:
- Adeno-associated Virus Vector Design Service
CD BioSciences has been committed to using genes for the treatment of human diseases through engineered AAV vectors. Based on our carefully constructed gene therapy platform, we now provide a series of specific AAV vector design services for laboratory research and clinical applications.
- Adeno-associated Virus Vector Purification Service
CD BioSciences laboratory has produced many advanced AAV vector purification methods. Generally, purification of AAV vectors by gradient centrifugation analysis and affinity chromatography analysis can shorten the turnaround time and produce reproducible concentrations.
- Adeno-associated Virus Production Service
CD BioSciences first subclone the target gene (GOI), shRNA or gRNA into the relevant pAAV cis plasmid (optional).
Large-scale preparation of pAAV cis and complementary plasmids.
The involved plasmids were large-scale transfected into 40x15cm plates of HEK293 cells.
The AAV producing cells were harvested and purified by a series of CsCl centrifugation.
Determine the titer of virus stock by quantitative real-time PCR (in units of genome copies per milliliter or GC/ml).
- Titration service of AAV vectors
These comprehensive services can meet the needs of customers in basic research and pre-clinical applications.
Our Advantages
- CD BioSciences diversified expression cells are available for selection, scientific and reasonable screening system, optimization of the best experimental conditions.
- The success rate of CD BioSciences experiments is as high as 95%. With a stable experimental system and rich experience, it will save you a lot of time and money.
- CD BioSciences can simultaneously express and purify 20L-1000L to provide you with milligrams to grams of protein.
- CD BioSciences from gene synthesis, protein expression and purification, to the subsequent supporting experimental platform.
Our Service Items
| Project name | Adeno-associated virus (AAV) service |
|---|---|
| Our service | Adeno-associated Virus Vector Design Service Adeno-associated Virus Vector Purification Service Adeno-associated Virus Production Process Service Titration Service of AAV Vectors |
| Features |
|
| Product delivery mode | We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report. |
| Price | Inquiry |
Why choose us?
CD BioSciences provides professional adeno-associated virus (AAV) service, which can meet the needs of regular customers for hit identification on time and on budget. Our service is a personalized and customized innovative scientific research service. Our experienced and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each adeno-associated virus (AAV) project. Before determining the corresponding analysis plan and price, each project needs to be evaluated. If you want to know more about service prices or technical details, please feel free to contact us.
