Glycosylation is the process of adding sugars to proteins or lipids under the control of enzymes, which occurs in the endoplasmic reticulum and Golgi apparatus. Under the action of glycosyltransferase, the sugar is transferred to the protein, and the amino acid residues on the protein are covalently bonded. Proteins undergo glycosylation to form glycoproteins. Glycosylation is an important modification of protein, and it can regulate protein function. Glycosylation can significantly influence protein stability, biological activity, and pharmacokinetics of the final products.
Lectin affinity method is currently a widely used method of separation and enrichment in glycoproteomics. Lectin is a type of sugar-binding protein that can specifically recognize and bind to a specific glycosyl sequence in a monosaccharide or glycan with a special structure. They are reversibly non-covalently bound to sugar chains, glycoproteins or After the glycopeptide is captured by the lectin, a specific monosaccharide is usually used to elute the glycoprotein or glycopeptide by competing with the lectin. After the protein is digested by enzymes, lectin is used to enrich N-glycosylated peptides, and then the sugar chains connected to asparagine residues (Asn) are excised in H218O with N-glycamidase (PNGase). This treatment caused an increase in the molecular weight of Asn by 2.9890 Da. Finally, a high-precision LC-MS mass spectrometer was used to detect the peptide after deglycation to confirm the change of the molecular weight and the theoretical molecular weight of the desaccharide and the sequence of the glycosylation modified peptide to determine the N-glycosylation site of the protein.
In vitro galactosylation and sialylation pilot analysis
In vitro glycoengineering scale up
In vitro galactosylation and sialylation optimization analysis
✓ Fast process, high protein recovery rate
✓ Stable and reliable glycan conversion by IVGE
✓ Extensive expertise in sugar analysis and other biological analysis capabilities
✓ Special analysis tools and equipment for evaluating IVGE success and data verification
- CD BioSciences flexibly and comprehensively provide all services related to glycosylation service.
- Our research supervisor and project manager will complete your project on time through open communication channels and meet your requirements.
- CD BioSciences has established a professional after-sales service team to provide customers with efficient and practical solutions.
Our Service Items
|Project name||Glycosylation service|
|Our services||CD BioSciences offers glycosylation service service to meet the specific needs of different customers.|
- 5 mg of purified protein at≥2 mg/mL
- Sequence of target protein
- Theoretical pI value
- Purification method/tag on protein of interest
|Product delivery mode||
We will complete the project on time according to the agreed time. CD BioSciences offer you the following results:
- Glycan Profiles
- Aggregation analysis report
- Purity analysis report
- Charge Variant Analysis report
Why choose us?
CD BioSciences provides professional glycosylation services to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class protein expression platform and technical expertise, we provide customers with the best quality one-stop glycosylation services, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.