Endotoxin Removal and Testing Services
CD BioSciences endotoxin removal and detection service is an important service item of prokaryotic protein expression and purification service. Endotoxin is widely present in the cell wall of Gram-negative bacteria (such as E.coli). Escherichia coli will produce a large amount of endotoxin in the process of expressing recombinant protein. As a heat source, endotoxin can cause inflammation and fever in organisms. Therefore, when recombinant protein is used in some animal experiments, endotoxin removal is required to avoid adverse reactions in animals and affect experimental results.
• General process of endotoxin removal
Endotoxin control and removal are based on the principle of endotoxin-binding peptide purification resin. It needs to be controlled from the beginning of the protein preparation experiment. All the utensils, instruments, water, reagents, pipettes and pipettes used in the experiment are all internal Toxin removal. At the same time, the experiment is completed in a clean room to ensure a very low endotoxin level.
• Endotoxin testing method
The endotoxin detection methods used by CD BioSciences include semi-quantitative gel detection and absolute quantitative coloration. The color method and gel method based on limulus reagent are currently the most accurate detection methods in the world.
✓ Efficient removal of endotoxin---guaranteed endotoxin level is less than 10EU/mg.
✓ Multiple strategies to solve the endotoxin problem, the success rate is as high as 98%.
✓ One-stop service from gene to low endotoxin protein.
✓ Estimate the protein loss rate at the beginning of the project to ensure that sufficient low endotoxin protein is provided.
Our Service Items
|Project name||Endotoxin removal and testing services|
✓ Evaluate the project & establish the plan
✓ Evaluate the target protein
✓ Evaluation of endotoxin removal efficiency and protein loss rate
✓ Target protein customized solution
✓ Endotoxin removal
✓ Use customized processes to remove endotoxins
✓ Endotoxin quantitative detection
✓ Quantitative detection before and after endotoxin removal
✓ Limulus reagent detection
|Product delivery mode||
We will complete the project on time according to the agreed time. CD BioSciences offer you the following results:
1. All purified protein
2. Study report
3. COA including CE-SDS analysis
Why choose us?
CD BioSciences provides professional endotoxin removal and testing servicess to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class protein expression platform and technical expertise, we provide customers with the best quality one-stop endotoxin removal and testing servicess, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.