Recombinant Adenovirus Construction Service
Adenovirus is a non-enveloped virus with an icosahedral protein capsid composed of a linear double-stranded DNA genome. Since the adenovirus vector cannot integrate into the host genome, multiple administrations are required. The side of the adenovirus vector is the ITR, and the inside of the ITR is the virus packaging signal. Four early transcription elements (E1, E2, E3, E4) are distributed on the genome to regulate replication. The most commonly used and most typical adenovirus vectors are types 5 and 2 based on human type C. CD BioSciences provides various types of adenovirus vectors with different designs and structures for customers to choose from. The packaging capacity and toxicity of the intestinalless are better than adenovirus vectors.
CD BioSciences provides customers with customized solutions and a series of recombinant adenovirus construction service, including but not limited to:
- Adenovirus constuction services
In CD BioSciences, PEG and pHPMA are used to modify adenoviral vectors to maintain efficient gene transfer and overcome obstacles that limit clinical applications. Different Ad serotypes made by various construction methods can be applied to pancreatic cancer, neuroendocrine cancer, breast cancer and glioblastoma.
- Construction of recombinant adenovirus in bacterial system
In bacterial systems, no ligation steps are required to generate plasmids, and any region of the adenovirus genome can be easily modified. In short, first clone the full-length adenovirus genome flanked by unique restriction enzyme sites into a bacterial plasmid. Next, the virus genome region to be modified is subcloned into a bacterial shuttle plasmid, and the required changes are introduced through molecular biology techniques.
- Regulating the construction of adenovirus vectors
The use of tissue-specific and cell-specific promoters to control gene expression has been extensively tested as a means of targeting transgene expression. Here, we can construct adjustable adenovirus vectors to help artificially control gene expression in vitro and in vivo.
- Construction of adenovirus/retrovirus hybrid vector
CD BioSciences offer hybrid adenovirus/retroviral vector systems designed to combine the advantages of adenovirus and retroviral vectors. In this system, the non-integrating adenovirus vector is functionally integrated through the intermediate production of retrovirus producing cells. Chimeric vectors can allow the realization of the necessary goals for specific gene therapy applications.
Our Service Items
|Project name||Recombinant adenovirus construction service|
|Product delivery mode||We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.|
Why choose us?
CD BioSciences provides professional recombinant adenovirus construction service, which can meet the needs of regular customers for hit identification on time and on budget. CD BioSciences relies on world-class viral vector technology expertise, we provide the highest quality one-stop recombinant adenovirus construction service for gene delivery, including the design and construction of adenovirus vectors according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.