Large-scale Protein Expression and Purification
CD BioSciences has a mature protein expression system and advanced protein expression technology. A large amount of protein is a prerequisite for scientific research, but there are still certain difficulties in large-scale protein production. We provide large-scale recombinant protein preparation services including: protein fermentation, high-density fermentation technology and process optimization.
CD BioSciences has various types of fermentation tanks, bioreactors, and protein purification systems, which can provide recombinant proteins in milligrams to grams. Customers can choose the appropriate expression system and fermentation program according to their needs.
Overall Solutions
| Service content | Fermentation amount | Deliverable | Cycle | Price |
|---|---|---|---|---|
| Protein fermentation | 5-50mg/L |
Protein Expression Bacteria Mud Experimental report Inspection report |
1 week | Inquiry |
| High density fermentation | 20-400mg/L | Inspection report | 3 weeks | Inquiry |
| Process optimization | 1000-1000mg/L |
High purity recombinant protein Detailed experiment data report Inspection report |
8-12weeks | Inquiry |
Service process
Gene optimization: gene sequence is a key factor in determining the success or failure of protein expression
Optimization items include: mRNA structure, rare codons, ribosome binding sites, etc.
Optimization of expression conditions: Optimization of protein expression conditions, including temperature, inducer, OD and other factors affecting protein expression, has achieved higher cell culture density and protein expression during subsequent fermentation.
Large-scale fermentation production: divided into ordinary protein fermentation and high-density fermentation, including protein fermentation process optimization, temperature, pH, and dissolved oxygen feeding methods.
Protein purification: After obtaining a large amount of protein expression bacterial paste, we can provide corresponding protein purification services (optional), a variety of protein purification methods to choose, and finally deliver the protein of the purity required by the customer.
Our Advantages
- The team of CD BioSciences has a wealth of knowledge and experience. We work with many from all over the world, and our service quality is guaranteed.
- Our research supervisor and project manager will complete your project on time through open communication channels and meet your requirements.
- CD BioSciences has established a professional after-sales service team to provide customers with efficient, fast and practical solutions.
- The proteins we deliver are stored in Tris buffer by default. If customers have requirements for protein storage conditions, we can also provide other buffers or freeze-drying services.
Why choose us?
CD BioSciences provides professional large-scale protein expression and purifications to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class protein expression platform and technical expertise, we provide customers with the best quality one-stop large-scale protein expression and purifications, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
