Fluorescent labeling is the process of combining fluorescent dyes with functional groups contained in biomolecules so that they can be visualized by fluorescent imaging. Fluorescent labels have many advantages because they are highly sensitive, long-term stable, and do not interfere with the function of the target molecule even at low concentrations. Targeted imaging of labeled cells can track them in vitro and in vivo. Using different fluorophores in the same sample can also observe multiple molecules at the same time. The most commonly used fluorophores are fluorescein isothiocyanate (FITC), rhodamine derivatives (TRITC), coumarin, and cyanine. These synthetic organic dyes are used to label biomolecules such as proteins, peptides, antibodies, nucleic acids, bacteria or yeast. Naturally occurring fluorescent dyes, such as green fluorescent protein (GFP), can also be used to genetically label living cells.
Fluorescent labeling technology
Chemical labeling technology: Fluorophores dock to the target molecule through chemical modification (covalent or non-covalent binding). Chemical labeling methods have many advantages because they are robust, easy to perform, and very effective, and are suitable for a wide range of fluorophores. They are more suitable for in vitro research rather than in vivo research.
Enzymatic labeling technology: Enzymatic reactions can be used for rapid, efficient and selective labeling in vivo and in vitro, and can be used to target proteins or whole cells.
Peptide/protein labeling: This technique is also easy to implement and can be used to study different sites of a single protein according to the specificity of the peptide tag.
Gene labeling: Gene tagging can be achieved by using fluorescent dye-labeled protein domains, small peptides or single amino acids. These amino acids can be specifically attached along chromosomes in the body. This method allows the detection of chromosomal abnormalities, such as deletions or duplications.
CD BioSciences is committed to providing high-quality fluorescent labels to major biopharmaceutical companies. As a life science service expert around you, we can provide one-stop or highly customized services according to the specific requirements of customers. Please contact us directly and we will get back to you as soon as possible. We can provide you with the following fluorescent labeling service not limited to:
▪ Fluorescent protein labeling
▪ Fluorescent labeling of nucleic acids
▪ Fluorescent labeling of polysaccharides
▪ Fluorescent labeling of lipids
Our service items
|Project name||Fluorescent labeling|
CD BioSciences offers fluorescent labeling to meet the specific needs of different customers.
According to your scientific research needs.
- CD BioSciences attaches great importance to innovation, the combination of production and R&D, and the introduction of high-end scientific research talents.
- The core R&D team of CD BioSciences has a multidisciplinary background, including medicine, pharmacy, biology, materials science and other R&D backgrounds. They have active innovative thinking, rich biological expertise and experimental research experience.
- CD BioSciences specializes in cellular immunofluorescence assay service and we are committed to contributing to more customers and we can provide all services related to cellular immunofluorescence assay service.
Why choose us?
CD BioSciences provides professional fluorescent labeling to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop fluorescent labeling, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more information, our scientists will tailor the most reasonable plan for your project.