Capsid Content Analysis Service

During the production of viral vectors, there exists a population of capsids that have failed to package the vector DNA. These empty capsids can represent up to 90% of the crude harvest for rAAV vector preparations. In addition, incomplete encapsidation is also observed, leading to capsids containing truncated vector genomes or capsids that contain illegitimate, non-vector DNA (from plasmids, cells, or helper viruses). While their impact on the efficacy of gene therapy is not fully understood, these capsids are considered an undesirable quality attribute. They are a source of unnecessary, potentially antigenic material, which could elicit an unwanted immune response in the patient. For example, in a clinical study for hemophilia B, capsid-specific cytotoxic T lymphocytes were implicated in an adverse immune response after successful expression of human coagulation factor IX using recombinant AAV2-mediated gene transfer. Therefore, the capsid content (ratio of empty to full capsids) is an important attribute to monitor in viral vector preparations.

Overall solution

CD BioSciences offers the following capsid content analysis methods to meet the specific needs of different customers.

AUC: Analytical ultracentrifugation (AUC) is a powerful tool to distinguish and quantify different AAV species by either mass (sedimentation velocity) or density (sedimentation equilibrium).

TEM (cryoTEM): Cryogenic TEM is a method where the biological specimens are vitrified by rapid freezing in liquid ethane thereby preserving their native structure.

Our advantages

  • First-class expert technical team: The team is professional and experienced.
  • The team of CD BioSciences has a wealth of knowledge and experience. We work with many from all over the world, and our service quality is guaranteed.
  • Our research supervisor and project manager will complete your project on time through open communication channels and meet your requirements.
  • CD BioSciences has established a professional after-sales service team to provide customers with efficient, fast and practical solutions.

Our service items

Project name Capsid content analysis service
Our services
  • CD BioSciences offers capsid content analysis service to meet the specific needs of different customers.
Product delivery mode We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.
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Why choose us?

CD BioSciences provides professional capsid content analysis service to meet the needs of regular customers to identify hits on time and on budget. CD BioSciences relies on world-class cell engineering technology expertise, we provide customers with the best quality one-stop capsid content analysis service, including the development of experimental programs according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

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