Cell Cycle Analysis

The cell cycle is the process by which eukaryotic cells duplicate and divide. The cell cycle consists of two specific and distinct phases: interphase, consisting of G1 (Gap 1), S (synthesis), and G2 (Gap 2), and the mitotic phase; M (mitosis) (Figure 1). During interphase, the cell grows (G1), accumulates the energy necessary for duplication, replicates cellular DNA (S), and prepares to divide (G2). At this point, the cell enters the M phase, which is divided into two tightly regulated stages: mitosis and cytokinesis. During mitosis, a parent cell's chromosomes are divided between two sister cells. In cytokinesis, the division of the cytoplasm occurs, leading to the formation of two distinct daughter cells. Each phase of the cell cycle is tightly regulated, and checkpoints exist to detect potential DNA damage and allow it to be repaired before a cell divide. If the damage cannot be repaired, a cell becomes targeted for apoptosis. Cells can also reversibly stop dividing and temporarily enter a quiescent or senescent state; G0. The first checkpoint is at the end of G1, making the decision if a cell should enter S phase and divide, delay division, or enter G0. The second checkpoint, at the end of G2, triggers mitosis if a cell has all the necessary components. CD BioSciences can provide you with professional cell cycle analysis to meet the specific needs of different customers.

Overall solution

By measuring the cell cycle, it can be used as a biocompatibility evaluation index, or used to study cell apoptosis, and can also be used as a basis for selecting cells. At present, the commonly used cell cycle detection method is PI staining detection. The DNA content in the cell is different in each period of the cell cycle. The fluorescent dye propidium iodide (PI) can bind to the DNA in the cell. The amount of the fluorescent dye combined with the different amount of DNA is different, so the fluorescence intensity detected by the flow cytometer It is also different, it can be used to reflect the status of each phase of the cell cycle, that is, the status of cell proliferation. CD BioSciences has an experimental team composed of researchers, and relying on the detection platform, it can provide cell cycle detection services for domestic scientific researchers.

Our service items

Project name Cell cycle analysis
Our services

CD BioSciences offers cell cycle analysis service to meet the specific needs of different customers.

Material Required

According to your scientific research needs, provide suitable nanomaterials or modification needs. For cell strains, please use dry ice or ice bags to transport samples.

Deliverables

Experimental raw data, pictures, analysis results; experimental process and a complete report.

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Our advantages

  • CD BioSciences will complete your project on time and efficiently. We have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
  • CD BioSciences attaches great importance to innovation, the combination of production and R&D, and the introduction of high-end scientific research talents.
  • CD BioSciences specializes in cell cycle analysis and we are committed to contributing to more customers and we can provide all services related to cellular immunofluorescence assay service.
  • CD BioSciences cooperates with scientists from many pharmaceutical and biotechnology companies. We have a wealth of knowledge and experience to provide quality assurance services.

Why choose us?

CD BioSciences provides professional cell cycle analysis to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop cell cycle analysis, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more information, our scientists will tailor the most reasonable plan for your project.

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