Sequencing of Antibody Variable Regions

CD BioSciences provides sequencing and cloning of antibody variable regions. Our antibody sequencing service can accurately deliver antibody heavy and light chain sequence information for you. With the antibody sequence, genetic engineering can be used for recombinant expression, the production cost is lower, and the batch difference is easier to control; at the same time, the modification and design of the antibody becomes feasible.

Overall solutions process

Sequencing of Antibody Variable Regions
  • CD BioSciences has developed an efficient and reliable method to sequence antibody variable regions (VH and VL) in hybridoma cell lines or clonal primary B cells of various species.
  • CD BioSciences performs RT-PCR on mRNA isolated from hybridoma samples and uses unique species-specific primer pairs to amplify the target region. Antibody Variable Region Sequencing (AVS) is a reliable service of CD BioSciences, and more than 5000 sequences have been identified. More than 95% of the samples have been successfully identified, and at least one heavy chain and one light chain have been identified.
  • Even with technically difficult samples, the CD BioSciences method has shown a high success rate in many cases. The combination of CD BioSciences' AVS service and automatic data tracking and management system provides customers with absolute confidence in every step.
  • After successfully cloning the variable region, the sequence can be fused to various IgG backbones. Alternatively, the variable region sequence can be reformatted into various configurations of single chain Fv (scFv) or bispecific antibodies. Downstream analysis services can then be used to verify and identify transiently produced antibodies.

Our service items

Project name Sequencing of antibody variable regions
Our service Cloning and sequencing of antibody variable regions:
1. Extract and purify total RNA from hybridoma cells or cloned B cells.
2. Use optimized primer combinations to form cDNA.
3. Use nested PCR to amplify DNA fragments corresponding to VL and VH.
4. Sequencing the amplified DNA fragments. Amplified PCR fragments of variable heavy and light chain sequences, plus shipping/handling fees.
Product delivery mode 1. Research report containing sequence analysis.
2. Amplified PCR fragments of variable heavy chain and light chain sequences are available
Sample requirements For cloning and sequencing of antibody V regions:
A. There are more than 100,000 cells in the frozen vial and shipped on dry ice;
B. The isolated RNA sample is dried on the RNA separation column (before elution) and shipped on dry ice.
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What we offer?

  • Cloning and sequencing of the variable region of mouse hybridoma IgG
  • Cloning and sequencing of rat hybridoma IgG variable region
  • Cloning and sequencing of rabbit hybridoma IgG variable region
  • Cloning and sequencing of rabbit hybridoma IgG variable region
  • Cloning and sequencing of primate B cell IgG variable region
  • Cloning and sequencing of hamster hybridoma IgG variable region
  • Cloning and sequencing of IgM variable region of mouse hybridoma
  • Cloning and sequencing of rat hybridoma IgM variable region
  • Cloning and sequencing of IgM variable regions of primate B cells

Why choose us?

CD BioSciences provides professional sequencing of antibody variable regions services, which can meet the needs of regular customers for hit identification on time and on budget. Sequencing of antibody variable regions service is a personalized and customized innovative scientific research service. Our experienced and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each Sequencing of antibody variable regions service project. Before determining the corresponding analysis plan and price, each project needs to be evaluated. If you want to know more about service prices or technical details, please feel free to contact us.

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