De Novo Antibody Sequencing Services
CD BioSciences is equipped with the most advanced technologies, such as high-performance liquid chromatography (LC-MS/MS) coupled with tandem mass spectrometry and advanced calculation algorithms. CD BioSciences can provide accurate and rapid antibody de novo sequencing services according to your needs. CD BioSciences' services include variable regions, variable regions and leader regions, as well as full-length heavy and light chain antibody sequencing for all species, isotypes and allotypes. The purified multivalent form of monoclonal antibodies can be sequenced with 100% coverage of the desired area and excellent accuracy. Many successful cases from CD BioSciences have confirmed our qualifications and can provide antibody sequencing with 100% accuracy and satisfaction to meet customer needs.
Overall Solutions
Based on our proprietary technology for antibody de novo sequencing and our expertise in the field of protein mass spectrometry and informatics, CD BioSciences' standard service procedures include the following steps:
- De novo antibody sequencing work flow chart 1 De novo antibody sequencing work flow.
- First use various trypsins to digest antibody proteins into overlapping peptides
- LC-MS/MS digestion, then fragment optimization
- The feature of overlapping peptides is de novo peptide sequencing
- Automated antibody sequence assembly using characterized peptides
- CD BioSciences use the most advanced calculation algorithm for data analysis, which can effectively obtain the amino acid sequence of the digested peptide
Our Advantages
- Customizable antibody fragment sequencing
We can choose to measure only the light chain, heavy chain or variable region of the antibody according to customer needs. In addition, sequencing of rare antibody subtypes such as conjugated antibodies and IgM can also be customized.
- Low time spent
Technological innovation has brought about a reduction in time cost. The time cost of the DASS platform is less than half of that of traditional sequencing methods. After receiving the samples, the full sequence determination can be completed within 3-10 working days.
- Low sample volume requirements
Only need to provide 25-100μg purified antibody.
- Diverse types of sequencing
It can sequence fluorescently labeled monoclonal antibodies, IgMs, and other non-traditional antibodies.
Our Service Items
| Project name | De Novo antibody sequencing services |
|---|---|
| Our services |
CD BioSciences offers de novo antibody sequencing services to meet the specific needs of different customers. |
| Sample requirements |
Only need to provide 25-100μg purified antibody. |
| Product delivery mode |
We will complete the project on time according to the agreed time. |
| Timeline |
1 to 2 weeks |
| Price | Inquiry |
Why choose us?
CD BioSciences provides professional de novo antibody sequencing services to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences provides customers with the best quality antibody humanization, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
