Virus Vector Purification Service
Gene therapy is the transfer of genetic material to the patient's cells to achieve a therapeutic effect. Most of the therapeutic DNA is delivered using viral vector systems based on adenovirus (Ad), adeno-associated virus (AAV) and lentivirus (LV). With the increase in the use of these viral vectors as clinical therapeutics, scalable commercial processes (especially purification) are being studied and optimized to ensure the maximum retention of critical quality attributes (CQA).
Types of viral vectors
Each type of viral vector has its own challenges and opportunities in gene therapy. Adenovirus was first used in gene therapy 20 years ago.
Lentiviruses have shown promise in clinical trials. They have been used to treat Wiskott-Aldrich syndrome, X-linked adrenal leukodystrophy and metachromatic leukodystrophy. The important mechanism of action (MoA) of lentiviruses is their ability to transduce dividing and non-dividing cells, resulting in sustained transgene expression and potential therapeutic effects.
- Adeno-associated virus (AAV)
AAV is also considered to be a promising gene therapy tool because they are not pathogenic and immunogenic, and have the characteristics of continuous transgene expression and suitable for various cells.
CD BioSciences offers the following virus vector purification service services to meet the specific needs of different customers.
|Purity||Host cell-related Impurities||
Host cell DNA/RNA: DNA Threshold assay, qPCR,
Host cell proteins: ELISA, TEM
|Capsid content (empty: full capsids)||TEM, AUC|
- Adenovirus purification service
The purpose of adenovirus purification is to obtain a pure adenovirus vector, which is convenient for subsequent scientific research or clinical treatment. The key to the purification of adenovirus is to remove or destroy host cell DNA with the help of nucleases. The scientific and technical team of CD BioSciences has established an adenovirus-centric technology platform to provide you with professional adenovirus purification services.
- Adeno-associated virus vector purification service
CD BioSciences has put in a lot of effort to determine the purification of AAV vectors. At present, our laboratory has produced many advanced AAV vector purification methods. CD BioSciences purified the AAV vector through gradient centrifugation analysis and affinity chromatography analysis to reduce turnaround time and produce reproducible concentrations. CD BioSciences can provide high-quality laboratory-grade AAV vectors in a short time.
- Lentiviral vector purification service
Lentiviral vectors (LVs) have recently witnessed increasing demand for research and clinical applications. The goal of CD BioSciences is to provide customers with high-quality lentiviral vector purification services to improve high yield and reduce impurity content.
Our service items
|Project name||Virus vector purification servicee|
|Product delivery mode||We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.|
Why choose us?
CD BioSciences provides professional virus vector purification service to meet the needs of regular customers to identify hits on time and on budget. CD BioSciences relies on world-class cell engineering technology expertise, we provide customers with the best quality one-stop virus vector purification service, including the development of experimental programs according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.