Hybridoma Antibody Gene Sequencing

Hybridoma antibody gene sequencing is based on RACE (Rapid Amplification Of cDNA Ends) technology. RACE technology is an effective method to rapidly amplify the 5'and 3'ends of cDNA from low-abundance transcripts by using PCR. It is widely used because of its simplicity and speed. CD BioSciences has rich RACE technology experience and can provide fast and reliable hybridoma cell sequencing. We use RACE technology to perform 5'-end RACE on monoclonal antibody genes, which can sequence the light chain, heavy chain, and full length of the antibody. The sequencing range covers mouse, rat, rabbit, sheep, chicken and other species. At the same time, CD BioSciences has a mature antibody preparation platform and can also provide recombinant antibody expression services. Now, CD BioSciences offers professional hybridoma antibody gene sequencing to meet the specific needs of different customers.

Overall solution

• Variable region sequencing

By designing specific primers and using PCR to amplify and sequence the antibody heavy chain and light chain variable regions, CD BioSciences finally obtains the heavy chain variable region (VH) and light chain variable region (VL) sequences.

• Full-length antibody sequencing

The monoclonal antibody gene was amplified by PCR method to obtain the complete sequence of the antibody heavy chain and light chain, and then the amplified cDNA was cloned into the vector and sequenced to obtain the full-length antibody sequence. In addition, CD BioSciences can also use the method of antibody subtype identification to obtain the constant region sequence of the antibody and sequence the variable region of the antibody to obtain the sequence of the variable region, and finally obtain the complete antibody full-length sequence.

Our advantages

▪ Reliable sequencing results

The delivered sequence of CD BioSciences can be smoothly used for recombinant expression, and its affinity is equivalent to that of the parent antibody.

▪ Rigorous scientific research attitude

CD BioSciences selects more than 5 positive clones for sequencing to eliminate the interference of false genes.

▪ A wide range of sequencing objects

CD BioSciences' sequencing targets cover hybridoma monoclonal antibodies from mouse, rat, rabbit, sheep, chicken and other species.

▪ Comprehensive service content

CD BioSciences provides hybridoma preparation, recombinant antibody preparation, interaction analysis and other related services.

Why choose us?

CD BioSciences provides professional hybridoma antibody gene sequencings service to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop hybridoma antibody gene sequencings service, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more CD BioSciencesled information, our scientists will tailor the most reasonable plan for your project.

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