CHO Transient Expression System

CHO transient expression system is dissociated plasmids or DNA fragments in cells that can be expressed in large quantities in the early stages of transient transfection. This is also an increasingly important method for rapid production of recombinant proteins in cells. Since the approval of the first CHO-derived therapeutic protein (tissue plasminogen activator, tPA) in 1986, the CHO cell line is the most widely used mammalian system for transient expression and production of recombinant proteins. CHO cells have always been the main force in protein and antibody production. The main advantages of CHO include strong growth, tolerance of suspension culture, production and secretion of stellar protein, and a long history. The biggest disadvantage of CHO is that the cells are difficult to transfect. Therefore, CHO cells have been mainly used for stable production of CHO, rather than transient production. Our technology platform solves the technical problem of low transient transfection efficiency. In the shake flask, we can achieve a yield of more than 1 gram per liter. CD BioSciences chose CHO as HEK293 as its transient protein production platform.

Overall solution

  • CD BioSciences chose CHO as HEK293 as its transient protein production platform. CHO transient expression system is the first choice for the production of biopharmaceuticals for pre-project evaluation and pre-clinical evaluation.
  • As a global leader in the field of drug development and manufacturing, CD BioSciences provides our valued customers with customized CHO transient expression systems to comprehensively evaluate the developability of various biological candidates.

Our advantages

▪ According to different needs, CD BioSciences can use a transient and stable CHO expression system to ensure comprehensive, repeatable results and timely delivery.

▪ CD BioSciences will complete your project on time and efficiently. We have professional after-sales service: a professional after-sales service team has been established to provide customers with efficient, fast, practical and feasible solutions.

▪ CD BioSciences' experienced project team will be assigned to each study, focusing on completing the progress of the project in the shortest possible time. Our customers widely regard us as a professional and attentive partner that provides quality results.

Our Service Items

Project name CHO transient expression system
Details

1. CHO cells can be scaled up to 0.03 to 10 liters to produce milligrams to grams of substances.

2. Purified antibodies will be obtained using the purification methods listed.

Customer needs to provide the following materials:

1. Antibody sequence

2. DNA ready for transfection

Deliverables

1. Purified protein

2. Study report

3. Certificate of Analysis

Timeline

3-4 weeks

Price Inquiry

Why choose us?

CD BioSciences provides professional CHO transient expression systems to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop CHO transient expression systems, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

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