Lentiviral Vectors Construction Service
Lentiviral vectors can effectively integrate exogenous genes or exogenous shRNA into the host chromosome, so as to achieve the effect of persistent expression of the target sequence. For some difficult-to-transfect cells, such as primary cells, stem cells, undifferentiated cells, etc., the use of lentiviral vectors can greatly improve the transduction efficiency of the target gene or target shRNA, and the target gene or target shRNA can be integrated into the host cell. The probability of the genome is greatly increased, and the long-term and stable expression of the target gene or target shRNA can be achieved more conveniently and quickly.
The HIV-1 vector is the most studied lentivirus and can be reproduced without expressing any helper genes. Lentiviral vectors usually contain only three HIV-1 genes: gag, pol and rev. In addition to the necessary cis-acting sequences (including LTR and packaging signals), the cloning vector does not contain all viral sequences. The rev response element (RRE) is included to ensure effective nuclear export of the full-length viral RNA genome. The use of vesicular stomatitis virus G protein (VSV-G) to replace the HIV envelope not only expands the types of cells that the vector can infect, but also produces a higher vector titer and results in a higher stability of the vector virus particle. CD BioSciences provides a variety of lentiviral vector targeting optimization methods, including direct targeting, tissue selective targeting, the use of tissue-specific promoters or miRNA-mediated silencing to control transgene expression.
Our service items
|Project name||Lentiviral vectors construction service|
|Product delivery mode||We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.|
Why choose us?
CD BioSciences provides professional lentiviral vectors construction service, which can meet the needs of regular customers for hit identification on time and on budget. CD BioSciences relies on world-class viral vector technology expertise, we provide the highest quality one-stop lentiviral vectors construction service for gene delivery, including the design and construction of lentiviral vectors according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.