The non-human antibodies entering the human body can cause severe rejection. For example, the entry of murine monoclonal antibodies into the human body will cause the human anti-mouse antibody response (HAMA response), which will affect the clinical application of antibodies and severely impair the safety and treatment effect. Antibody humanization is the process of genetic engineering and recombinant expression of animal-derived antibodies so that most or all of the antibody's sequence becomes human-derived, ensuring antibody affinity and specificity and minimizing the heterogeneity of antibodies to humans.
Antibody sequencing, structural biology analysis, phage display system, cell display system, protein expression purification system, Biacore/Fortebio test platform, stable cell line construction.
- IgG: CD BioSciences uses cell surface display technology to obtain humanized IgG antibodies by constructing and screening humanized IgG antibody mutation libraries.
- Small-molecule antibodies: CD BioSciences uses phage display technology to display humanized antibody mutation libraries on the surface of phage, and screen to obtain high-affinity and high-specificity humanized small-molecule antibodies.
✓ Project experience: A number of antibody humanization and affinity maturation projects have been completed, and many modified antibodies are already in the clinical research stage.
✓ Humanization of antibodies from multiple species: including dogs, monkeys, mice, camels, alpacas, chickens, etc.
✓ Using the means of bioinformatics and structural biology, computer-aided design of antibody modification.
✓ The degree of humanization is relatively high. After the transformation, after the affinity maturation process, the affinity can reach nM and pM levels.
✓ Various humanization methods: chimeric antibody, CDR grafting, SDR grafting, chain replacement.
Our Service Items
|Project name||Antibody humanization|
CD BioSciences offers the following antibody humanization services to meet the specific needs of different customers.
✓ Antibody sequence annotation
✓ Antibody sequence analysis
✓ Antibody structure prediction and optimization
✓ Humanized antibody design, immunogenicity prediction.
✓ Antibody-antigen interaction prediction
✓ Design and modification of antibody molecules
1. Parental antibody VL and VH sequences
2. DNA template (if DNA synthesis is not desired)
3. Target Antigen
|Product delivery mode||
We will complete the project on time according to the agreed time. We provide you with the following results:
1. Top three antibodies based on antigen binding affinity.
2. 100 ug purified antibody for the three top clones.
3. Study report.
4 to 6 months
Why choose us?
CD BioSciences provides professional antibody humanization to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality antibody humanization, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.