What is karyotype analysis?
Karyotype analysis refers to the process of artificially pairing, numbering and grouping the chromosomes of the cell to be tested according to the inherent chromosome morphological and structural characteristics of the organism and according to certain regulations, and performing morphological analysis. The chromosomes of different species have their own specific morphological structure (including the number of chromosomes, length, centromere position, arm ratio, satellite size, etc.), and this morphological feature is relatively stable. The dyed or fluorescently labeled chromosomes can clearly and intuitively observe the specific morphological structure of the chromosomes through certain optical or electrochemical color development equipment, and then compare with the normal karyotype to find differences, and then determine the deletion, duplication and chromosome phenomena such as inversion.
- Routine morphological analysis.
- Band analysis.
- Colored segment analysis.
We swode elect the chromosomes in the metaphase of mitosis of the vigorously dividing cells to make a karyotype map to determine the length (μm) or relative length (%) of each chromosome, the centromeric position and the ratio of the two arms of the chromosome (arm ratio), and identify the The presence or absence of body and accessory constriction serves as the basis for analysis.
G-banding technology is to color different regions of chromosomes by Giemsa staining method, so that the chromosomes show light and dark bands under a light microscope. Each chromosome has a specific band pattern, and even the long and short arms of each chromosome are specific. According to the different banding types of chromosomes, the individuality of chromosomes can be identified more carefully and accurately.
The chromosomes are processed by low temperature, KCl and enzymatic hydrolysis, HCl or HCl and acetic acid mixed liquid, and then processed into slices, which can cause the heteropyknosis reaction of the chromosomes and make the heterochromatin segments visible. The coloring segments are basically the same between homologous chromosomes, but there are differences between non-homologous chromosomes.
Karyotype analysis is a cytogenetic test that can identify numerical and structural chromosomal abnormalities. CD BioSciences uses conventional staining techniques to color condensed chromosomes with G for observation. The chromosomes are then grouped according to chromosome size, centromere position and banding pattern, and any potential aberrations are identified.
CD BioSciences can process BSL1 and BSL2 samples. In order to receive and process living cells classified as BSL2, we need the following screening evidence:
- Confirm by PCR or equivalent test that the sample material does not contain human pathogens from CLIA- (HBV, HCV, HIV-1, HIV-2, HTLV I/II, CMV, EBV, HSV-1, HSV-2) certification test room.
- Confirm that the sample does not contain mycoplasma by PCR or similar methods.
- Mature cell technology platform: Many years of cellular experience
- First-class expert technical team: The team is professional and experienced.
- Professional after-sales service: A professional after-sales team is set up to provide customers with practical and feasible solutions efficiently and quickly.
Our service items
|Project name||Karyotype analysis|
CD BioSciences offers karyotype analysis to meet the specific needs of different customers.
|Product delivery mode||We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report. The service result includes a descriptive report and at least one high-quality image of a representative karyotype.|
Why choose us?
CD BioSciences provides professional karyotype analysis to meet the needs of regular customers to identify hits on time and on budget. CD BioSciences relies on world-class cell engineering technology expertise, we provide customers with the best quality one-stop karyotype analysis, including the development of experimental programs according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.