Antibody Affinity Maturation
Antibodies are widely used in the diagnosis and treatment of various diseases. Affinity maturation is the process of manipulating the variable regions of antibodies that have undergone somatic hypermutation to introduce point mutations in the framework and CDR loops to select variants with improved binding affinity for their target. Achieving high-affinity binding is essential to amplify the dissociation half-life, expand the detection limit, reduce the drug dose, and improve the efficacy of the drug. However, in vivo antibody affinity maturation usually fails to produce antibody drugs with targeted efficacy. Therefore, it is necessary to carry out further affinity maturation in vitro through directed evolution. CD BioSciences has established a comprehensive antibody design platform. We provide high-quality Antibody affinity maturation services based on our advanced platform, and we will do our best to promote the success of your project.
Overall Solutions
In CD BioSciences, phage and yeast surface display platforms are used for different antibody affinity maturation methods, and then high-throughput (HT) screening is performed to improve target binding affinity.
1. CDR randomization of different Ab fragment scaffolds (scFv, Fab, VHH).
2. Large single or large combination variable heavy/light chain yeast or phage display CDR libraries can be generated.
3. Multiple rounds of panning selections with increasing stringency can be performed in order to achieve demanding affinity improvements CD BioSciences can generate light chain shuffled antibody libraries by amplifying the VL library of B cells from human donors, including yeast or phage display libraries.
Our Advantages
✓ CD BioSciences team has worked with scientists from many pharmaceutical and biotechnology companies and organizations around the world. We have a wealth of knowledge and experience in antibodies.
✓ CD BioSciences will complete your project efficiently and on time, and meet your requirements.
✓ CD BioSciences has professional after-sales service: a professional after-sales service team has been established to provide customers with efficient, fast, practical and feasible solutions.
Our Service Items
| Project name | Antibody affinity maturation |
|---|---|
| Our services |
CD BioSciences offers the following antibody humanization services to meet the specific needs of different customers. |
| Sample requirements |
1. Sequence of VH and VL. 2. DNA template (if DNA synthesis is not desired). 3. Target Antigen |
| Product delivery mode |
We will complete the project on time according to the agreed time. We provide you with the following results: 1. Top three antibodies based on antigen binding affinity. 2. 100 ug purified antibody for the three top clones. 3. Study report. |
| Timeline |
4 to 6 months |
| Price | Inquiry |
Why choose us?
CD BioSciences provides professional antibody affinity maturation to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality Antibody affinity maturation, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
