Lentiviral Vectors Titration Service
Lentiviral vectors are effective vectors for stable gene transfer in dividing and non-dividing cells (such as neurons or hepatocytes), and allow long-term expression of transgenes and target specific cell types through a simple pseudotyping process. Based on these advantages, in molecular research and preclinical/clinical trials, LV is often used to deliver genes of interest to mammalian cells. However, in order to apply the lentiviral method to research and clinical research, it is first necessary to evaluate the potency. Virus titer determination is an important quality assessment based on the basic research and clinical application of lentivirus, especially the accuracy of the assessment is critical to the patient's therapeutic dose.
- CD BioSciences lentivirus titration can be determined by two broad categories: non-functional (physical titer) and functional (infectious titer) methods.
- CD BioSciences provides non-functional titrations, including the use of enzyme-linked immunosorbent assay (ELISA) to detect expressed viral proteins (such as p24) or transcripts thereof, to evaluate reverse transcriptase (RT) activity, and to determine the concentration of genomic RNA in vector preparations.
- CD BioSciences provides a functional titration method that can detect integrated proviral DNA, transgenic mRNA or protein expression levels in target cells. The titration method based on protein expression is usually based on the expression of the reporter gene encoded by the vector or staining with an antibody against the transgene.
Our service items
|Project name||Lentiviral vectors titration service|
|Product delivery mode||We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.|
Why choose us?
CD BioSciences provides professional lentiviral vectors titration service, which can meet the needs of regular customers for hit identification on time and on budget. CD BioSciences relies on world-class viral vector technology expertise, we provide the highest quality one-stop lentiviral vectors titration service for gene delivery. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.